Tethered-bilayer lipid membranes as a support for membrane-active peptides.
نویسندگان
چکیده
An immunosensing device, comprising a lipid membrane incorporating ion channels tethered to the surface of a gold electrode, has been reported [Cornell, Braach-Maksvytis, King, Osman, Raguse, Wieczorek and Pace (1997) Nature (London) 387, 580-583]. The present article describes key steps in the assembly of the device and provides further evidence for its proposed sensing mechanism.
منابع مشابه
The assembly and use of tethered bilayer lipid membranes (tBLMs).
Because they are firmly held in place, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cornell et al. Nature 387(6633): 580-583, 1997). Here we describe the procedures required to assemble and test tethered lipid bilayers that can incorporate various lipid species, peptides, and ion channel proteins.
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 29 Pt 4 شماره
صفحات -
تاریخ انتشار 2001